1. komponenta

Lecture type	Total
Lectures	45
Practicum	30
Seminar	30

* Load is given in academic hour (1 academic hour = 45 minutes)

1. Introduction, kinds of macromolecules, thermodynamics of chemical reactions
2. Water, chemical bonds, pH, buffers
3. Amino acids, peptides, primary and secondary structures of proteins
4. Tertiary and quaternary structures of proteins, denaturation and renaturation of proteins
5.Methods in protein chemistry- isolation and characterisation of peptides and proteins, determination of 3D structure of proteins
6. Functions of proteins-functions of fibrous proteins, functions of globular proteins- interactions of proteins with their specific ligands: haemoglobin and myoglobin, antibody-antigen interactions
7. Enzymes (introduction)-enzyme catalysed reactions, thermodynamics of enzyme catalysed reactions
8. Enzyme kinetics (Michaelis-Menten) and kinds of inhibitors
9. Mechanisms of enzyme action and regulation of enzyme activity
10. Carbohydrates-monosaccharides, disaccharides, polysaccharides, glycoproteins and proteoglycans
11. Lipids- fatty acids, triacylglyceroles, membrane lipids, sterols, waxes
12. Biological membrane, structure and function
13. Transport of ions and small molecules through membranes
14. Signal transduction in eukaryotic cells
15. Introduction to metabolism- transfer of phosporyl groups, electrons, acyl groups, types of the most frequent metabolic reactions
16. Glycolysis and regulation of glycolytic pathway
17. Gluconeogenesis and regulation of gluconeogenesis
18. Pentose phosphate pathway and regulation of the pathway
19. Glycogen-degradation and regulation of blood level glucose
20. Glycogen synthesis and control of the glycogen synhesis
21. Synthesis of acetyl-CoA- pyruvate dehydrogenase complex
22. Citric acid cycle
23. Transport of electrons in mitochondria-generation of electrochemical gradient
24. Proton-motive force and ATP synthesis
25. Photosynthesis
26. Calvin cycle and biosynthesis of monosaccharides and polysaccharides
27. Degradation of fatty acids
28. Synthesis of fatty acids
29. Synthesis of lipids-synthesis of acylglyceroles, phospholipids and cholesterol
30. Urea cycle
31. Degradation of essential amino acids
32. Degradation of non-essential amino acids
33. Fixation of nitrogen-nitrogenase complex
34. Synthesis of amino acids
35. Synthesis of porphyrins, heme, chlorophylls, synthesis of some neurotransmitters
36. Synthesis and degradation of nucleotides
37. Integration of metabolic pathways
38. Structures of DNA and RNA
39. DNA replication
40. DNA repair and recombination
41. RNA synthesis and regulation of RNA synthesis in prokaryotes
42. Gene expression in eukaryotes
43. Maturation of eukaryotic RNA
44. Genetic code
45. Mechanisms of protein synthesis

- to describe structures of proteins, polysaccharides and nucleic acids, and to expain their functions in the cell
- to describe the structure and function of biological membranes and to explain transport systems in the membranes
- to understand and explain basics metabolic pathways
- to explain and differentiate biosynthetic pathways of macromolecules (carbohydrates, lipids, proteins, nucleic acids)
- to determine isoelectric point of amino acids
- to perform experiment of enzyme kinetics and to determine Michaelis-Menten constant
- to separate individual proteins from the mixture of proteins
- to understand and describe how the energy in the cell is obtained and stored
- to understand and to explain how thermodynamics is coupled to chemical reactions in the cell

1. J. M. Berg, J. L. Tymoczko, L. Stryer. Biokemija, Školska knjiga 2014. (prijevod 6. Izdanja J. M. Berg, J. L. Tymoczko, L. Stryer. Biochemistry, 6. izdanje, W. H. Freeman Co. 2008)
   J. M. Berg, J. L. Tymoczko, L. Stryer. Biochemistry, 7. izdanje, W. H. Freeman Co. 2012
   D. L. Nelson, M. M. Cox, Lehninger Principles of Biochemistry, 6. izdanje, W. H. Freeman Co. 2013.
   J. L. Tymoczko, J. M. Berg, L. Stryer. Biochemistry, A short course, W. H. Freeman Co. 2010.
   B. Mildner, predavanja i seminari iz "Osnove biokemije"
2. R. K. Murray, D. A. Bender, K. M. Botham, P. J. Kennely, V. W. Rodwell, P. A. Weil, Harperova iliustrirana biokemija, 28. izdanje, Medicinska naklada, Zagreb, 2011.

Enrollment :
Passed : Cell and Molecular Biology
Passed : Organic Chemistry

Mandatory course - Regular study - Biology