COURSE CONTENT:
Introduction to the analytical procedure, basics of quality assurance and validation with respect to key performance parameters (selectivity, specificity, accuracy, precision, limit of detection and quantification). Analytical method versus analytical procedure. Reporting of results. Repeating of elemental statistical methods (mean, median, standard deviation, statistical tests). Basics on modern analytical chemistry, techniques and instrumentation. Atomic spectrometry, elemental analysis of real samples (food, soils, water). Photospectrometry, application on biomolecules (amino acids), acid-base indicators, chromophores, metal complexes. Fluorescence spectroscopy: application on biological samples, biomolecules, FRET, FISH, green fluorescent protein. Mass spectrometry for determining the elemental composition of environmental samples, geochemistry, proteomics. Nuclear magnetic resonance for medical diagnosis and determination of biomolecule structure (proteins, nucleic acids, protein-DNA/RNA complexes, oligosaccharides). Electroanalytical methods, application of biosensors and an ion-selective electrodes for various components (ions, oxygen, carbon dioxide, cholesterol, glucose, urease, creatine kinase). Immunochemical methods: application in biology, agronomy, forensics, pharmacy. Enzyme-immunoassays for the determination of drugs, narcotics, vitamins and biological fluids. Separation methods (chromatography, extraction, electrophoresis, ultracentrifugation). Separation methods and hyphenation methods for purifying and analysis of food ingredients, narcotics, alcohol, lipids, nucleic acids and proteins.
LEARNING OUTCOMES:
- define accurate and precise analytical analysis and apply basic statistical methods to assess analytical measurement data quality
- demonstrate understanding on the working principle of different analytical techniques and recognize their advantages and limitations
- select the most adequate analytical techniques on the basis on the kind of compounds they are trying to quantifycorrectly describe the principles of sampling and applications of traditional and modern sample pretreatment prior to analysis - storage, drying, etc.
- correctly describe the basic working principle of different separation methods, including extraction techniques, and chromatography
- explain the basic working principles and applications of different chromatographic methods (planar and column liquid chromatography, high performance liquid chromatography, adsorption, ion-exchange, affinity, and gas chromatography
- correctly describe the applications of electrophoresis and centrifugation to the study of DNA and proteins
- explain the basic working principles and applications of spectroscopic methods including absorption spectroscopic methods, fluorescence methods, atomic absorption, inductively coupled plasma optical emission, inductively coupled plasma mass spectrometry,
- correctly describe aplication of nuclear magnetic resonance determination of a molecules structure (proteins, nucleic acids) and for medical diagnosis
- correctly explain use of mass spectrometry for determination of a molecules structure (vitamins, steroids, drugs, proteins, nucleic acids)
- explain the basic application of electrochemical methods for biomolecules including potentiometry, biosensors, and an ion-selective electrodes
- correctly describe the use of enzymes (as reagens or in enzyme-immunoassays methods) for real samples (food, drugs, vitamins)
- explain the basic principles and applications of immunological methods in biology, forensic, pharmaceutical, and environmental science
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- 1. D. A. Skoog, D. M. West, F. J. Holler: Osnove analitičke kemije, prijevod: N. Kujundžić, V. Allegretti Živčić, A. Živković, Školska knjiga, Zagreb 1999.
2. D. A. Skoog, J. F. Holler, S. R. Crouch, Principles of Instrumental Analysis, 6. izd., Thomson, Belmont, 2007.
3. Highschool books (Ministry of Science)
4. J. M. Erwing, J. G. Houba, Plant Analysis Procedures, 2nd ed., Kluewer Academic Pub., London, 2004.
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