Introduction to the analytical procedure, basics of quality assurance and validation with respect to key performance parameters (selectivity, specificity, accuracy, precision, limit of detection and quantification). Analytical method versus analytical procedure. Reporting of results. Repeating of elemental statistical methods (mean, median, standard deviation, statistical tests). Basics on modern analytical chemistry, techniques and instrumentation. Atomic spectrometry, elemental analysis of real samples (food, soils, water). Photospectrometry, application on biomolecules (amino acids), acid-base indicators, chromophores, metal complexes. Fluorescence spectroscopy: application on biological samples, biomolecules, FRET, FISH, green fluorescent protein. Mass spectrometry for determining the elemental composition of environmental samples, geochemistry, proteomics. Nuclear magnetic resonance for medical diagnosis and determination of biomolecule structure (proteins, nucleic acids, protein-DNA/RNA complexes, oligosaccharides). Electroanalytical methods, application of biosensors and an ion-selective electrodes for various components (ions, oxygen, carbon dioxide, cholesterol, glucose, urease, creatine kinase). Immunochemical methods: application in biology, agronomy, forensics, pharmacy. Enzyme-immunoassays for the determination of drugs, narcotics, vitamins and biological fluids. Separation methods (chromatography, extraction, electrophoresis, ultracentrifugation). Separation methods and hyphenation methods for purifying and analysis of food ingredients, narcotics, alcohol, lipids, nucleic acids and proteins.
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