1. komponenta

Lecture type	Total
Lectures	15
Practicum	30

* Load is given in academic hour (1 academic hour = 45 minutes)

COURSE OBJECTIVES

To gain knowledge and skills in laboratory bioassay systems, interpretation of results and evaluation of intensity and mechanism of response to toxic effects.

COURSE CONTENT
LECTURES
Definition of bioassays: laboratory toxicity tests; overview of bioassays and their standard procedures. Test organisms and cells (main characteristics, selection of the test organisms or cells). Laboratory animals and animals collected from the nature, native animals (laboratory care and maintenance).
Categorization of bioassays depending on the organism used: bacterial bioassays (Microtox, Mutatox, Ames test, Umu test etc.), yeast bioassays, protozoan bioassays, phytoplankton bioassays (growth inhibition test of the green algae etc.), zooplankton bioassays (Daphnia test etc.), plant bioassays (Allium test, Lemna test, seed germination test, etc.).
Categorization of bioassays depending on the mechanism of toxicity/end points: lethal and sublethal bioassays, animal behaviour tests, growth and development inhibition bioassays, genotoxicity bioassays (genetic and chromosomal mutations, micronucleus-test and comet assay, sister chromatid exchange, alkaline elution of DNA, tests for detection of apoptosis, RAPD method etc.), bioassays of inhibition/induction of enzymes (mixed function oxidases, glutathione-S-transferase etc.), bioassays of cell viability, bioassays of proliferation of cell lines, bioassays of macromolecular damage, bioassays of cell organelles (mitochondrial respiratory inhibition etc.).

LABORATORY EXERCISES
Comet test
Micronucleus test
Cytotoxicity test
Plant bioassay systems
Polymorphic DNA markers: RAPD method
qPCR method: expression analysis

1. Interna skripta s predavanjima i protokolima praktikuma.
2. Maluszynska J., Plewa, M., (ed.) 2003: Bioassays in Plant Cells for Improvement of Ecosystem and Human Health. Wydawnictwo Uniwersytetu Slaskiego, Katowice.
3. Analitika okoliša (2013) Ašperger D, Babić S, Bolanča T, Darbra RM, Ferina S, Ginebreda A, Horvat AJM, Kaštelan-Macan M, Klobučar G, Macan J, Mutavdžić Pavlović D, Petrović M, Sauerborn Klobučar R, Štambuk A, Tomašić V, Ukić Š. Hinus, Zagreb, str. 294
4. Moriarty, F., 1990: Ecotoxicology. The study of pollutants in ecosystems. 2nd ed. Academic Press, London.
5. Rand, G. M., Petrocelli, S. R., 1985: Fundamentals of Aquatic Toxicology. Metods and Applications. Hemisphere Publishing Corporation, Washington.

Enrollment :
Attended : Genetics
Attended : Plant Physiology

Izborni predmeti - Regular study - Molecular Biology