Karlo Miškec, mag. biol. mol.

Zvanje:	stručni suradnik
Lokacija:	Borongajska cesta 83H, BIOCentar
E-mail:	E-mail
Osobna stranica na Webu:	https://www.linkedin.com/in/karlo-mi%C5%A1kec-324392173/?originalSubdomain=hr
Zavod/služba:	Zavod za molekularnu biologiju
Godina magistriranja:	2019.
Na zavodu od:	2020.

Nastava

preddiplomski

• Genetika (240544) - Vježbe u praktikumu
• Kultura animalnih i biljnih stanica (40880) - Vježbe u praktikumu
• Kultura animalnih i biljnih stanica (40887) - Vježbe u praktikumu
• Kultura animalnih i biljnih stanica (kolegij izvođen na engleskom jeziku) (172450) - Vježbe u praktikumu

diplomski

• Kultura animalnih i biljnih stanica (246024) - Vježbe u praktikumu

Konzultacije

Po dogovoru, kontaktirati mailom.

Životopis

I'm a molecular biologist with two years of experience in working with plant tissue culture, bacterial and yeast strains. During my internship and Master's thesis internship I've mostly worked with recombinant protein expression and have mastered molecular methods such as Western blot, pull down, yeast two hybrid, X-Gal test, PCR, molecular cloning, optimizations of protein expression and solubility and other methods. I have great experience in plant tissue cultivation and manipulation as well as in bacterial and yeast cultivation.

Currently I am working as a PhD student in Laboratory of Genetics and Epigenetics under the mentorship of Ph.D, assoc. prof. Aleksandar Vojta where I am researching the mechanism of IgG Fc chain glycosylation pathway and potential GWAS hits for glycosylation with epigenetic CRISPR/dCas9 modular system in suspension stable monoclonal cell lines HEK293-FreeStyle with dCas9/VPR or dCas9/KRAB domains stably integrated in cells genome as well as in suspension cell line LCL. Currently we are developing a new in vivo system of gene manipulation with CRISPR/dCas9 system in mouse strains C57BL/6 with lentiviral technology.

Izabrane publikacije

1. A Transient Expression System with Stably Integrated CRISPR-dCas9 Fusions for Regulation of Genes Involved in Immunoglobulin G Glycosylation

Authors:
Anika Mijakovac, Karlo Miškec, Jasminka Krištić, Vedrana Vičić Bočkor, Vanja Tadić, Maria Bošković, Gordan Lauc, Vlatka Zoldoš, Aleksandar Vojta

Publication date: 2022/1/12

Journal: The CRISPR Journal

Publisher: Mary Ann Liebert, Inc., publishers

2. Effects of Estradiol on Immunoglobulin G Glycosylation: Mapping of the Downstream Signaling Mechanism

Authors
Anika Mijakovac, Julija Jurić, Wendy M Kohrt, Jasminka Krištić, Domagoj Kifer, Kathleen M Gavin, Karlo Miškec, Azra Frkatović, Frano Vučković, Marija Pezer, Aleksandar Vojta, Peter A Nigrović, Vlatka Zoldoš, Gordan Lauc

Publication date:  2021/5/25

Journal: Frontiers in immunology

Volume: 12

Pages: 1922

3. BPM1 regulates RdDM-mediated DNA methylation via a cullin 3 independent mechanism

Authors:
Mateja Jagić, Tamara Vuk, Andreja Škiljaica, Lucija Markulin, Vedrana Vičić Bočkor, Mirta Tokić, Karlo Miškec, Genadij Razdorov, Siniša Habazin, Marko Šoštar, Igor Weber, Nataša Bauer, Dunja Leljak Levanić

Publication date:  2022/9/6

Journal: Plant Cell Reports

Publisher: Springer Berlin Heidelberg

Pages: 1-19

Profesionalni interesi i članstva

Hrvatska udruga genetičkih inženjera - HUGI.

Izabrani projekti

KK.01.1.1.04.0085 Genomsko inženjerstvo i genska regulacija u staničnim linijama i modelnim organizmima tehnologijom CRISPR/Cas9 - CasMouse 20.12.2019. - 31.12.2022.

Voditelj projekta: prof.dr.sc Vlatka Zoldoš