1. komponenta

Lecture type	Total
Lectures	20
Seminar	10

* Load is given in academic hour (1 academic hour = 45 minutes)

The types of chromatography analytical techniques: relative to aggregate states of stationary and mobile phase, performance, and physicochemical processes. Theory of chromatographic processes. Factors that influence chromatographic processes. Separation efficiency and selectivity. Generating of chromatographic analytical data and data processing. Analyte characterization and quantitative analysis of specific mixture components. Selection of chromatographic method relative to analyte chemical properties. Sample preparation prior to chromatographic analysis.
Liquid Chromatography (LC): Stationary and mobile phase properties. Adequate instrumental and detection techniques for specific analyte analysis. High Performance Liquid Chromatography: liquid adsorption chromatography, partition liquid chromatography. Normal- and reversed-phase chromatography. Ion-pair chromatography. Chiral chromatography. Ion-exchange chromatography. Size exclusion chromatography. Multidimensional chromatography and hyphenated instrumental techniques. Chromatographic separation and protein mixture analysis. Applications.
Gas Chromatography (GC): Selection of suitable mobile and stationary phases. Gas-liquid chromatography. Adsorption chromatography. Design of gas chromatography system. Multidimensional chromatography and hyphenated instrumental techniques. Applications.
Supercritical fluid chromatography. Properties of mobile and stationary phases. Design of chromatography system.
Capillary electrophoresis. Principle. Adequate instrumental and detection techniques. Capillary electrochromatography. Micellar electrokinetic chromatography. Applications.
description of instruction methods Lectures, seminars, laboratory demonstrations, consultations
description of course/module requirements Written and oral exam

1. OBVEZNA LITERATURA:
   P. Worsfold, A. Townshend, C. Poole (ur.): Encyclopedia of Analytical Science, Second Edition, Elsevier Ltd., Oxford 2005.
   M. Kaštelan-Macan, M. Medić-Šarić, S. Turina: Plošna kromatografija, Farmaceutsko-biokemijski fakultet Sveučilšta u Zagrebu, Zagreb 2006.
   I. Piljac: Elektroforeza, Media Print, Zagreb 2006.
   R. E. Ardrey: Liquid Chromatography-mass spectrometry: An introduction, John Wiley & Sons Ltd., Chichester 2005.
   M. Kaštelan-Macan, M. Petrović (ur.):Analitika okoliša, Hinus&Fakultet kemijskog inženjerstva i tehnologije Sveučilišta u Zagrebu, Zagreb, 2013.
   L. T. Taylor, Supercritical Fluid Chromatography, Anal. Chem. 82 (2010)4925-4935.
   J. V. Seeley, S. K. Seeley, Multidimensional Gas Chromatography: Fundamental Advances and New Applications, Ana.l Chem. 85 (2013)557-578.
2. DOPUNSKA LITERATURA:
   Suvremeni opsežniji revijski članci i znanstvene monografije

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